Posted inGenetic Engineering Updates
What are the differences between PCR, RT-PCR, qPCR, and RT-qPCR?
Polymerase chain reaction (PCR) is a relatively simple and widely used molecular biology technique to…
Posted inGenetic Engineering Updates
The role of PCR in single cell analysis
The polymerase chain reaction (PCR) has a wealth of applications in research, but perhaps one of its…
Posted inGenetic Engineering Updates
Colony PCR
Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally this…
Posted inGenetic Engineering Updates
What is Hot Start PCR?
Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the…
Posted inGenetic Engineering Updates
How to do ARMS-PCR?
The process of the ARMS PCR is simple and very effective. No harmful radiolabelling is…
Posted inGenetic Engineering Updates
What is ARMS-PCR or allele-specific PCR?
The allele-specific PCR also called as an ARMS- PCR (amplification refractory mutation system) or PASA…
Posted inGenetic Engineering Updates
Role of nested PCR in microbial identification
The nested qPCR or the nested RT-PCR gives great power to this technique which can…
Posted inGenetic Engineering Updates
What is nested PCR?
In the nested PCR, the specificity of the PCR reaction is enhanced by reducing the…
Posted inGenetic Engineering Updates
Protocol for inverse PCR
In the beginning, before doing any wet lab work, design the primers complementary to the…
Posted inGenetic Engineering Updates
Inverse PCR
Here, the unknown DNA sequence adjacent to the known template DNA is amplified using the…