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Posted inGenetic Engineering Updates
How to do ARMS-PCR?
The process of the ARMS PCR is simple and very effective. No harmful radiolabelling is…
Posted inGenetic Engineering Updates
What is ARMS-PCR or allele-specific PCR?
The allele-specific PCR also called as an ARMS- PCR (amplification refractory mutation system) or PASA…
Posted inGenetic Engineering Updates
Role of nested PCR in microbial identification
The nested qPCR or the nested RT-PCR gives great power to this technique which can…
Posted inGenetic Engineering Updates
What is nested PCR?
In the nested PCR, the specificity of the PCR reaction is enhanced by reducing the…
Posted inGenetic Engineering Updates
Protocol for inverse PCR
In the beginning, before doing any wet lab work, design the primers complementary to the…
Posted inGenetic Engineering Updates
Inverse PCR
Here, the unknown DNA sequence adjacent to the known template DNA is amplified using the…
Posted inGenetic Engineering Updates
Maxam-Gilbert Sequencing: What Was It, and Why
In the mid-1970s, two methods were developed for directly sequencing DNA: the Maxam-Gilbert sequencing (or…
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Clone Library Screening
The result of any cloning experiment that begins with total DNA from a specific source…
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Prokaryotic Expression Vectors
The first vector we will consider is the pUC family of vectors Figure 3.4.1: pUC vector Although it…
Posted inGenetic Engineering Updates
cDNA and Genomic Libraries
Isolation of corresponding genetic information Instead of synthesizing a desired gene, can we used the…